Objective From May 2017 to November 2021, there was an increased number of cases of fever with rashes admitted to Huoshan County Hospital in Lu’an City, Anhui Province, China. The aim of the study is to find out the etiology of the patients through epidemiological and clinical characteristic analysis, so as to provide a scientific basis for the prevention and control of the febrile illness with rashes. Methods An epidemiological investigation was carried out on the cases of fever with rashes through inquiring their medical histories and reviewing their medical records. The acute sera at admission and convalescent sera were collected from the patients to examine IgG antibody titers to Anaplasma, Borrelia burgdorferi, Orientia, Ehrlichia, Rickettsia rickettsii, and R. typhi using indirect immunofluorescence assay. An infection of a pathogen was determined by a 4-fold increase of the serum IgG antibody titer to the pathogen between the acute serum and the convalescent serum. The etiologic agent causing the outbreak was further identified by PCR amplification and DNA sequences of the rickettsial genes from acute serum. Results The IgG antibodies to Anaplasma, B. burgdorferi, and R. typhi were all negative in the acute sera of the patients. The positive rate of serum IgG antibodies to R. rickettsii was 51.52% (68/132) in the acute sera and 89.19% (33/37) in the convalescent sera. In the paired sera, the IgG titer had more than four time increase in the acute sera than in the convalescent sera. Phylogenetic analysis with the Rickettsia ompA sequence indicated that the etiologic agent formed a monoclade with newly identified Candidatus R. jingxinensis in Yunnan, China. Conclusion The febrile cases with rashes in Lu’an were serologically diagnosed as spotted fever rickettsial infection.

Objective To investigate the type distribution and molecular characteristics of Yersinia enterocolitica in Anhui province, China, 2018-2019. Methods Different types of samples were collected from patients with diarrhea, animal feces, and food from 2018 to 2019, Y. enterocolitica strains were isolated to perform systematic biochemical identification, serotyping, polymerase chain reaction detection of virulence genes, and molecular typing by pulsed-field gel electrophoresis (PFGE). Results Among 2 186 samples, a total of 97 strains of Y. enterocolitica were isolated, with O:3 (21/97, 21.65%) and O:5 (17/97, 17.53%) being the main serotypes. Virulence gene analysis showed that 19.59% (19/97) of the strains were attachment invasion locus gene-positive ( ail+), Yersinia stable toxin A gene-positive ( ystA+), Yersinia stable toxin B gene-negative ( ystB-), Yersinia adhesin A gene-positive ( yadA+), and yop regulon transcriptional activator gene-positive ( virF+); 63.92% (62/97) were ail-, ystA-, ystB+, yadA-, and virF-. Molecular typing by PFGE showed ≥ 95.00% similarity between the strains from patients with diarrhea and the strains from the host pigs. Conclusion In Anhui province, most Y. enterocolitica strains isolated from food, environment, and some patients with diarrhea are non-pathogenic. There are pathogenic strains in the host pigs and some patients with diarrhea. Strains isolated from pigs are closely related to human infection. Pigs are the main host of pathogenic strains of Y. enterocolitica.

【Abstract】 Objective To study the synergistic mechanism of synergistic rodenticides, and to observe the pathological changes in rodents tissue so as to provide the evidence for its application. Methods The effect of synergistic rodenticides to CT, PT, APTT and FIB were evaluated at the condition of SD rat as a model. The pathological changes in the tissues were detected by paraffin slice and HE staining technique. Results Single synergist could prolong CT and APTT and also increased FIB. While both warfarin and synergistic warfairn could obviously prolong CT, PT and APTT and reduced FIB. Compared to warfarin, the synergistic warfairn could increase CT, PT and APTT and reduced FIB. The histology and pathology observation indicated that synergistic warfairn could lead to a peptic ulcer compared with warfarin. It also led a pneumorrhagia and necrotic liver in toxic rats. Conclusion It suggests that synergist has an anticoagulant function, and the anticoagulant role of synergistic warfairn is powerful than that of traditional warfarin. Synergistic warfairn also leads to internal hemorrhaget and other physiological disfunction, while single synergist does not cause the histopathological damage.

Objective The research centers upon the ecological regulation and characters of the birds in Foshan Airport to advance feasible methods to avoid bird strike. Methods By the ecological environment of bird community distribution,fixed lines were chosen and investigated for one year at fixed time and data were analysized in terms of density,importance value and distributive coefficient. Results The birds were investigated in 10 kilometers square of Foshan Airport include 6 samples of 6 habitats,there were 49 species belonging to 10 orders and 23 families. An analysis of importance value indicated that 6 species,i.e. Ardeola bacchus, Egretta garzetta, Apus affinis, Hirundo rustica, Hirundo daurica, Pycnonotus sinensis,were the most important species. According to the habitat distributive coefficient of birds,16 species,which represented 32.7% of the whole species and constitute the main part of the bird communities in all the six habitats,were classified as cosmopolitan distribution pattern. Conclusion The analysis of birds distribution and that of time and space features has indicated that the major species creating bird strike are waders and other wetland birds and that spring (March to May) and autumn (September to November) are the seasons in which bird strike happens most frequently.